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Структуры хроматина сперматозоидов человека и фрагментация днк в норме и при нарушениях фертильности
Автор: Брагина Е., Арифулин Е., Хафизова П. и др   

 

magazine-vrach-2013-02-019

Структуры хроматина сперматозоидов человека и фрагментация днк в норме и при нарушениях фертильности

Основной метод обследования мужчин при бесплодии – изучение таких показателей спермы, как концентрация и количество, подвижность и мор- фология сперматозоидов. И хотя у фертильных мужчин эти показатели в целом выше, чем у инфертильных, возможно их существенное совпаде- ние, что позволяет предполагать наличие некоторых факторов, которые не учитываются при традиционном спермиологическом обследовании. Дополнительные тесты дают возможность исследовать функции компо- нентов сперматозоидов, ответственных как за собственно оплодотворе- ние, так и принимающих участие в эпигенетической регуляции раннего эмбриогенеза. В свете широкого внедрения в клиническую практику вспо- могательных репродуктивных технологий (ВРТ) особое внимание привле- кает структура собственно генетического материала – ядра сперматозоида (целостность ДНК, структура хроматина), аномалии которого могут приво- дить к бесплодию и неудачам ВРТ.
Human sperm chromatin structures and dna fragmentation in health and infertility
The main method for the examination of infertile males is to study the indicators of sperm, such as its concentration and amount, mobility and morphology. Although these indicators are generally higher in fertile men than in infertile ones, there may be their substantial coincidence, suggesting some factors that are ignored during traditional spermiological examination. Complementary tests provide a way of investigating the function of the sperm components responsible for both conception proper and involvement in the epigenetic regulation of early embryogenesis. In the light of the wide introduction of assisted reproductive technologies (ART) into clinical practice, of particular interest is the structure of the genetic material proper – the nucleus of a spermatozoon (structure, DNA fragmentation, anomalies of chromatin and its package), whose abnormalities may give rise to infertility and ART failures.

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